Donning a black p***yhat, the music icon caused controversy by dropping the F-bomb four times, sparking a slew of apologies from broadcasters airing the protest live.
She went on to speak of her rage at the election result, telling the crowd she had thought a lot about 'blowing up the White House' but knew that it 'wouldn't change anything'.
Tempers ran high as marchers took to Washington D.C. to oppose Donald Trump's new presidency - with Ashley Judd joining Madonna in spewing lewd rants against the new President.
The Hollywood actress and the pop star departed from the general spirit of inclusivity and calls for mutual respect with personal attacks not only on Trump but also his family, including daughter Ivanka.
'And to our detractors that insist that this march will never add up to anything, f*** you,' Judd proclaimed.
Madonna also performed two of her classic hits, Express Yourself and Human Nature, changing one of the lyrics in the latter song to 'Donald Trump suck a d***'.
Both CNN and MSNBC quickly cut away after Madonna's third f-word, with CNN's Brooke Baldwin apologizing for the expletives that they aired.
But C-SPAN stuck with their ongoing coverage of the march.
The pair were just two of the big names to speak at the march's rally at the National Mall for a sea of protesters in pink 'p***yhats', knitted beanies with car ears that have become the unofficial accessory of the march.
Among other notable figures to attend the March on Washington were Cher, America Ferrera, Scarlett Johanesson, Alicia Keys, and Amy Schumer.
On Sunday, Madonna that she was speaking metaphorically.
Madonna's speech, which was criticized on social media, led some television networks to abruptly stop their live feeds of the march, which drew hundreds of thousands of people in demonstrations across the United States to protest the election of Donald Trump as president.
"I am not a violent person," the singer songwriter said on Instagram. "I spoke in metaphor and I shared two ways of looking at things — one was to be hopeful, and one was to feel anger and outrage, which I have personally felt."
The 58-year-old led the crowd on Saturday in chants of, "Yes, we're ready" to take on policies promoted by Trump, who alienated many women during the election campaign with comments' about rivals' attractiveness and promises to outlaw or diminish abortion rights.